TaqMan real-time PCR: a reliable method to detect meat species
Authors
Abstract:
The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-time PCR using beef, lamb, pork, chicken, horse, and donkey target genes was performed. The results indicated that 100% of samples yielded positive results of beef, but none of them generated donkey and pork amplicons. The samples of Kebab indicated 93.33% lamb, 83.33% chicken, 10% horse positive results. The information claimed for the source of meat was not correct for more than 95% of the samples and only one food center labeled the Kebab mix correctly. Except for one Kebab sample that showed a positive result for the presence of horse meat, no sample contained meat of species considered illegal in Halal foods.
similar resources
PCR and real-time PCR assays to detect fungi of Alternaria alternata species.
Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morpholo...
full textA SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products.
Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye was proposed for the quantitative detection of pork meat in processed meat products. For the development of the method, binary meat mixtures containing known amounts of pork meat in p...
full textA TaqMan real-time PCR method based on alternative oxidase genes for detection of plant species in animal feed samples
Traceability of processed food and feed products has been gaining importance due to the impact that those products can have on human/animal health and to the associated economic and legal concerns, often related to adulterations and frauds as it can be the case for meat and milk. Despite mandatory traceability requirements for the analysis of feed composition, few reliable and accurate methods ...
full textDevelopment of a real-time fluorescence resonance energy transfer PCR to detect arcobacter species.
A real-time PCR targeting the gyrase A subunit gene outside the quinolone resistance-determining region has been developed to detect Arcobacter species. The species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. Discrimination between Arcobacter species was straightforward, as the corresponding melting points s...
full textThe Real-Time TaqMan PCR and Applications in Veterinary Medicine
The polymerase chain reaction (PCR), first described in 1985, is a highly sensitive and specific technique used for the detection of nucleic acids [55]. The inventor of this technology earned a Nobel prize for his achievement [43,44], which has revolutionised research and diagnostic possibilities. Qualitative PCR is a well established and straightforward technology, but quantification of specif...
full textConventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat
Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...
full textMy Resources
Journal title
volume 3 issue 2
pages 116- 120
publication date 2020-12-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023